Motivators of physical exercise in older adults from different contexts / Motivos de práctica de ejercicio físico en adultos mayores de diferentes contextos

The aim of the present study was to understand the reasons to join or to continue in a physical exercise programme based on the perceptions of the older adults participating in the study. Four focus groups were selected in various contexts, with a total of 39 older adults. An emergent induction analysis methodology was applied, handling the participants' speeches from the specific to the general through the coding process proposed in the grounded theory. The differences among contexts regarding personal reasons for physical practice were presented, as well as the role of physical and psychological benefits, and body image. The participants of this research engaged in physical exercise to pursue physical health benefits in the first place, followed by psychological well-being, and leaving body image aside (AU)

Este estudio se realizó para tratar de comprender cuáles son los motivos encontrados al unirse a programas o mantener la práctica de ejercicio físico en base a las percepciones de las personas adultas mayores que participan en el estudio. Se realizaron cuatro grupos focales en diferentes contextos con un total de 39 adultos mayores. El análisis metodológico es inductivo, emergente, delimitando los discursos de los participantes del estudio de lo específico a lo general, a través del proceso de codificación de la teoría fundamentada. Se presentaron las diferencias entre contextos sobre los motivos de carácter personal para la práctica física, el papel de los beneficios físicos, psicológicos y la imagen corporal. Los participantes de esta investigación realizan ejercicio físico buscando en primer lugar los beneficios para la salud física, seguido del bienestar psicológico y dejando de lado la mejora de la imagen corporal (AU)

Considerations after lockdown and overcoming Covid-19: A systematic review for returning to safe physical exercise practice.

Objective: This systematic review aims to understand the problems associated with lockdown and the various conditions of Covid-19 infection and to help prepare athletes and exercise enthusiasts for the safe resumption of sport in a manner that promotes wellness, healthy competition, and a sports industry that survives the current situation. This systematic review was carried out, following the recommendations of the currently pre-established reporting elements for systematic reviews and meta-analyses. The following databases were consulted: ISI Web of Science (WOS), Scopus and Google Scholar. This review includes a total of 19 articles. News: The results are presented based on three predominant themes: (1) psychological impact produced by SARS-CoV-2; (2) post-Covid-19 cardiac abnormalities and (3) protocols for an adequate return to physical practice. Prospects and projects: Most of the protocols shown by the various papers are consistent in terms of duration and number of phases. Based on this, the safe return to practice protocol can be divided into four stages of seven days in each phase in order to progress according to the symptoms perceived. In each phase, the physiological demand and efforts necessary to cover the proposed activities are increased until optimal physical condition is recovered.

Objectif: Cette revue systématique vise à comprendre les problèmes associés au confinement et aux diverses conditions d'infection au Covid-19 et à aider à préparer les athlètes et les amateurs d'exercice à la reprise du sport en toute sécurité d'une manière qui favorise le bien-être, une compétition saine et une industrie du sport qui survit à la situation actuelle. Cette revue systématique a été réalisée en suivant les recommandations des éléments de notification actuellement préétablis pour les revues systématiques et les méta-analyses. Les bases de données suivantes ont été consultées : ISI Web of Science (WOS), Scopus et Google Scholar. Cette revue comprend un total de 19 articles. Actualités: Les résultats sont présentés selon trois thèmes prédominants : (1) impact psychologique produit par le SARS-CoV-2 ; (2) anomalies cardiaques post-Covid-19 et (3) protocoles pour un retour adéquat à la pratique physique. Perspectives et projets: La plupart des protocoles présentés par les différents papiers sont cohérents en termes de durée et de nombre de phases. Sur cette base, le protocole de retour à la pratique en toute sécurité peut être divisé en quatre étapes de sept jours chacune afin de progresser selon les symptômes perçus. Dans chaque phase, la demande physiologique et les efforts nécessaires pour couvrir les activités proposées sont augmentés jusqu'à ce que la condition physique optimale soit retrouvée.

Lactoferrin gene polymorphisms associated with clinical mastitis in Honduran Holstein inheritance cows.

BACKGROUND: Lactoferrin (LTF) is an iron-binding glycoprotein found in milk and other exocrine secretion with antibacterial activity proposed as an alternative to mastitis treatment or prevention. LTF has been proposed as a candidate gene for mastitis resistance selection. The aim of this paper was to assess LTF promotor to explore variations with potential association to mastitis resistance in dairy cows from Honduras. METHODS: A resequencing of promotor and Exon I of LTF gene in extreme mastitis susceptibility cows (126 Holstein and Holstein crossbred) was performed. RESULTS: Eight polymorphisms were found in promotor region, four of them were novel variations. Two were important by frequency among extreme groups, but a polymorphism in - 421 A/T position was significantly (P = 0.0188) associated to mastitis susceptibility. CONCLUSION: Results support the key role of regulatory region of LTF gene. Some candidate genes are proposed in association with mastitis traits and implications are discussed.

Antimicrobial prophylaxis post-amniocentesis procedures in cattle: A randomized controlled equivalence study.

Amniocentesis is a routine procedure utilized on several species including human, equine, and bovine patients. Early assessment and discovery of new genetic traits in the cattle industry are highly desirable in order to accelerate genetic gain by shortening generational intervals. One of the main concerns from this procedure is the introduction of pathogenic bacterial contamination into the amniotic cavity thereby increasing the risks of spontaneous pregnancy losses post procedure. In this randomized controlled equivalence study, we have tested the effect of antimicrobial prophylaxis on the incidence of spontaneous abortions and contrasted it to untreated individuals post amniocentesis. On the treated group (n = 67) all heifers remained pregnant whereas 1 of the untreated group (n = 65) resulted in a spontaneous abortion during the study period. The latter represents 1.54% of pregnancy losses attributed to the risk associated to the amniocentesis procedure. However, the probability of inducing spontaneous abortion from the technique itself is not different to that of the contemporaneous population (n = 694) not undergoing amniocentesis viz., 1.59%. Following a two-tailed distribution, statistical analyses showed no significant differences across treatments (Fisher's exact test P = 0.49). The current prospective study indicates that performing amniocenteses on cattle have resulted in similar spontaneous pregnancy losses comparable to those of pregnant heifers without undergoing amniocentesis and regardless of antimicrobial use. In conclusion, prophylactic antimicrobials may not be applicable within the cattle amniocentesis framework.

Determining the probability of locating peaks using computerized peak-location methods in gamma-ray spectra as a function of the relative peak-area uncertainty.

The probabilities of locating peaks with a high relative peak-area uncertainty were determined empirically with nine types of peak-location software used in laboratories engaged in gamma-ray spectrometry measurements. It was found that it is not possible to locate peaks with a probability of 0.95, when they have a relative peak-area uncertainty in excess of 50%. Locating peaks at these relatively high peak-area uncertainties with a probability greater than 0.95 is only possible in the library-driven mode, where the peak positions are supposed a-priori. The deficiencies of the library-driven mode and the possibilities to improve the probabilities of locating peaks are briefly discussed.

Generation of ECG signals from a reaction-diffusion model spatially discretized.

We propose a model to generate electrocardiogram signals based on a discretized reaction-diffusion system to produce a set of three nonlinear oscillators that simulate the main pacemakers in the heart. The model reproduces electrocardiograms from healthy hearts and from patients suffering various well-known rhythm disorders. In particular, it is shown that under ventricular fibrillation, the electrocardiogram signal is chaotic and the transition from sinus rhythm to chaos is consistent with the Ruelle-Takens-Newhouse route to chaos, as experimental studies indicate. The proposed model constitutes a useful tool for research, medical education, and clinical testing purposes. An electronic device based on the model was built for these purposes.

Cultivar influence on variability in olive oil phenolic profiles determined through an extensive germplasm survey.

Despite the evident influence of the cultivar on olive oil composition, few studies have been devoted to exploring the variability of phenols in a representative number of monovarietal olive oils. In this study, oil samples from 80 cultivars selected for their impact on worldwide oil production were analyzed to compare their phenolic composition by using a method based on LC-MS/MS. Secoiridoid derivatives were the most concentrated phenols in virgin olive oil, showing high variability that was significantly due to the cultivar. Multivariate analysis allowed discrimination between four groups of cultivars through their phenolic profiles: (i) richer in aglycon isomers of oleuropein and ligstroside; (ii) richer in oleocanthal and oleacein; (iii) richer in flavonoids; and (iv) oils with balanced but reduced phenolic concentrations. Additionally, correlation analysis showed no linkage among aglycon isomers and oleocanthal/oleacein, which can be explained by the enzymatic pathways involved in the metabolism of both oleuropein and ligstroside.

[Evaluation of quality of life, social stigma and treatment adherence in patients with epilepsy in the Caceres Healthcare Area: a cross-sectional study]. / Valoracion de la calidad de vida, estigma social y adhesion al tratamiento en pacientes con epilepsia del Area de Salud de Caceres: estudio transversal.

INTRODUCTION: Epilepsy is a chronic disease with high prevalence. Its impact in the quality of life is influenced by factors like disease characteristics, comorbidities, stigma perception and treatment adherence. AIM: To assess the quality of life, the social stigma and the treatment adherence of patients with epilepsy in our area. PATIENTS AND METHODS: Descriptive, observational and cross-sectional study. Sociodemographic and clinical data were collected. We analyzed data on quality of life (QUOLIE-10), social stigma and treatment adherence through different questionnaires. RESULTS: 40 patients were studied. The mean age was 42.43 ± 17.20 years, and the onset of epilepsy was 25.88 ± 16.81 years. 55% were women. The score in the quality of life questionnaire was 19.28 ± 8.03 points in men and 17.00 ± 7.69 for women, this difference being not significant. There were also no significant differences for social stigma, with a mean of 11.50 ± 13.47 points in men and 6.00 ± 10.36 points for women (p = 0.152). According to the treatment adherence questionnaire (Morisky-Green), 70% of the patients studied were compliant with the treatment. CONCLUSIONS: Patients with epilepsy in our environment have a good quality of life, probably related to good treatment adherence and compliance. There is low perceived social stigma but people with epilepsy still trend to hide their condition.

TITLE: Valoracion de la calidad de vida, estigma social y adhesion al tratamiento en pacientes con epilepsia del Area de Salud de Caceres: estudio transversal.Introduccion. La epilepsia es una enfermedad cronica, con elevada prevalencia. La repercusion de la epilepsia en la calidad de vida de las personas que la padecen se ve influida por factores como las caracteristicas de la enfermedad, la presencia de comorbilidad, la percepcion de estigma social y la adhesion al tratamiento. Objetivo. Valorar la calidad de vida, el estigma social y la adhesion al tratamiento de pacientes con epilepsia de nuestra area. Pacientes y metodos. Estudio descriptivo, observacional y transversal. Se recogieron datos sociodemograficos y clinicos. Analizamos datos sobre la calidad de vida (Quality of Life in Epilepsy-10), el estigma social y la adhesion al tratamiento a traves de diferentes cuestionarios. Resultados. Se estudio a 40 pacientes, con una media de edad de 42,43 ± 17,2 años y una media de edad de inicio de la epilepsia de 25,88 ± 16,81 años. El 55% fueron mujeres. La puntuacion en el cuestionario sobre calidad de vida fue de 19,28 ± 8,03 puntos para los hombres y 17,00 ± 7,69 para las mujeres, y esta diferencia no fue significativa. Tampoco se observaron diferencias significativas para el estigma social: media de 11,50 ± 13,47 puntos para los hombres y 6,00 ± 10,36 para las mujeres (p = 0,152). Segun el cuestionario de adhesion terapeutica (Morisky-Green), el 70% de los pacientes fue cumplidor del tratamiento. Conclusiones. Los pacientes con epilepsia tienen una buena calidad de vida, que creemos atribuible a la buena adhesion y el cumplimiento del tratamiento. Se detecta una baja percepcion de estigma social, pero los pacientes tienden a ocultar el diagnostico.

Morphological evaluation of Day 8 embryos developed during induced aluteal cycles in the mare.

A novel in vivo model utilizing serial administrations of PGF2α to induce aluteal cycles in the mare was used to evaluate the effects of progesterone-deprivation on the morphology of in vivo preimplantation embryos. We hypothesized that equine embryos produced during induced aluteal cycles (AL) would be developmentally affected, characterized by earlier embryo stage at collection, smaller embryo diameter, and lower quality grade, compared with those collected on the same day post-ovulation from control cycles during diestrus (high progesterone; > 4 ng/mL). Seven cyclic mares with a median age of 6.5 years (range 3-16) were utilized in a crossover design. Mares in estrus were artificially inseminated to a fertile stallion and randomly assigned to control or AL groups. Mares received either saline solution (control mares) or PGF2α (AL mares), twice daily on days 0, 1, and 2 and once daily on days 3 and 4. Serial blood samples were collected daily during estrus and until the day of embryo collection 8 days after ovulation. Mares were monitored until they returned to estrus, and artificially inseminated. Mares were switched to the opposite treatment group only after a successful embryo collection occurred during the previous cycle. Only cycles that produced embryos were used for analyses. No significant rise in progesterone was observed in the AL group with mean concentrations of plasma progesterone remaining <1.0 ng/mL from ovulation until embryo collection on Day 8. This is in sharp contrast to the control (luteal) cycle where a post-ovulatory rise in plasma progesterone was observed. The mean daily concentrations of plasma progesterone were significantly higher in control vs. AL group beginning at Day 3 and remained so until Day 8. The mean (±SEM) embryo diameter of AL embryos was 171 ± 5 µm compared to 756 ± 99 µm for control embryos. The majority of the Day 8 AL embryos were classified as morulas (3/9) or early blastocysts (5/9) with only 2 embryos of quality grade 1 compared to the Day 8 control embryos that were mostly expanded blastocysts (6/7) with 5 of 6 being of quality grade 1. This study shows that serial administrations of PGF2α were able to prevent significant rises in plasma progesterone, thus inducing aluteal cycles characterized by a progesterone-deprived environment for developing embryos. Embryos collected from induced aluteal cycles were adversely affected as demonstrated by a lower quality grade, smaller diameter and earlier embryo stage at collection when compared to control embryos.

Wnt/ß-catenin activation and macrophage induction during liver cancer development following steatosis.

Obesity confers an independent risk for carcinogenesis. In the liver, steatosis often proceeds cancer formation; however, the mechanisms by which steatosis promotes carcinogenesis is unknown. We hypothesize that steatosis alters the microenvironment to promote proliferation of tumor initiating cells (TICs) and carcinogenesis. We used several liver cancer models to address the mechanisms underlying the role of obesity in cancer and verified these findings in patient populations. Using bioinformatics analysis and verified by biochemical assays, we identified that hepatosteatosis resulting from either Pten deletion or transgenic expression of HCV core/NS5A proteins, promotes the activation of Wnt/ß-catenin. We verified that high fat diet lipid accumulation is also capable of inducing Wnt/ß-catenin. Caloric restriction inhibits hepatosteatosis, reduces Wnt/ß-catenin activation and blocks the expansion of TICs leading to complete inhibition of tumorigenesis without affecting the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) loss regulated protein kinase B (AKT) activation. Pharmacological inhibition or loss of the Wnt/ß-catenin signal represses TIC growth in vitro, and decreases the accumulation of TICs in vivo. In human liver cancers, ontology analysis of gene set enrichment analysis (GSEA)-defined Wnt signature genes indicates that Wnt signaling is significantly induced in tumor samples compared with healthy livers. Indeed, Wnt signature genes predict 90% of tumors in a cohort of 558 patient samples. Selective depletion of macrophages leads to reduction of Wnt and suppresses tumor development, suggesting infiltrating macrophages as a key source for steatosis-induced Wnt expression. These data established Wnt/ß-catenin as a novel signal produced by infiltrating macrophages induced by steatosis that promotes growth of tumor progenitor cells, underlying the increased risk of liver tumor development in obese individuals.

Experiencias adversas de la infancia en una muestra de pacientes con enfermedad crónica en Cali-Colombia / Adverse childhood experiences in a sample of patients with chronic diseases in Cali, Colombia / Experiências adversas da infância em uma amostra de pacientes com doença crônica em Cali-Colômbia

Objetivo: evaluar la frecuencia de Experiencias Adversas de la Infancia (EAI) y las características sociodemográficas de la población con enfermedad crónica. Metodología: se condujo un estudio tipo corte transversal descriptivo, en el que se entrevistaron 121 pacientes mayores de 18 años de edad con enfermedades crónicas no transmisibles que acudieron a consulta externa de Medicina Interna en la Fundación Valle del Lili entre septiembre de 2015 y diciembre de 2015. A los participantes les aplicaron escalas validadas para evaluar EAI y otras variables clínicas de interés. Se realizó un análisis descriptivo de los datos. Resultados: de los participantes, el 69.4% estaba conformado por mujeres, la mediana de edad de los participantes fue 62 años con un RIQ de 50 a 72 años. Se encontró que el 64% de los encuestados presentó por lo menos un tipo de EAI, y el 23% presentó cuatro o más EAI y el abuso físico y la separación parental son los más frecuentes (32% y 23%, respectivamente). Conclusiones: las Experiencias Adversas de la Infancia son eventos prevalentes en nuestra población; sin embargo, no se les ha prestado la atención debida. Se necesitan más estudios para medir el impacto real a corto y a largo plazo, con las implicaciones respectivas para nuestro sistema de salud.

Objective: To evaluate the frequency of Adverse Childhood Experiences (ACEs) and the sociodemographic characteristics of the population with chronic disease. Methodology: Cross-sectional descriptive study that was conducted with 121 patients over age 18 with non-communicable, chronic diseases that sought internal medicine outpatient services at Fundación Valle del Lili from September to December of 2015. Validated scales to assess ACEs and other clinical variables of interest were applied to patients. Descriptive analysis of the data was conducted. Results: Of the participants, 69.4% was female; the median age was 62 years, with an IQR of 50 to 72 years. It was found that 64% of those surveyed presented at least one type of ACE, and 23% presented four or more ACEs, while physical abuse and parental separation were the most frequent (32% and 23%, respectively). Conclusions: Adverse Childhood Experiences are prevalent events in our population; however, such events have not received the attention needed. Further studies are warranted to measure the short- and long-term actual impact, with the respective implications for our health system.

Objetivo: avaliar a frequência de Experiências Adversas da Infância (EAI) e as características sócio-demográficas da população com doença crônica. Metodologia: se conduz um estudo tipo corte transversal descritivo, no qual se entrevistaram 121 pacientes maiores de 18 anos de idade com doenças crônicas não transmissíveis que procuraram a consulta externa de Medicina Interna na Fundación Valle del Lili entre setembro de 2015 e dezembro de 2015. Aos participantes lhes aplicaram escalas validadas para avaliar EAI e outras variáveis clínicas de interesse. Se realizou uma análise descritivo dos dados. Resultados: dos participantes, 69.4% estava conformado por mulheres, a idade média dos participantes foi de 62 anos com um RIQ de 50 a 72 anos. Se encontrou que 64% dos entrevistados apresentou pelo menos um tipo de EAI, e 23% apresentou quatro ou mais EAI e o abuso físico e a separação parental são os mais frequentes (32% e 23%, respectivamente). Conclusões: as Experiências Adversas da Infância são eventos prevalentes na nossa população; porém, não se há dado a atenção devida. Se necessitam mais estudos para medir o impacto real a curto e a longo prazo, com as implicações respectivas para nosso sistema de saúde.

Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro.

Paraoxonase-1 (PON1) is an enzyme found in serum and follicular fluid that protects cell membrane and circulating lipids against oxidative damage. The aims of this study were to measure the direct effects of recombinant PON1 (rPON1) on bovine oocyte maturation at the molecular level (gene expression) and to measure the carry-over effects of PON1 on pre-implantation embryo development in vitro. COCs were submitted to IVM with the addition of 0.0, 0.02, 0.04 and 0.08 mg ml(-1) of rPON1, corresponding to an average PON1 arylesterase enzyme activity of 2.2 ± 0.4, 15.5 ± 1.5, 30.2 ± 3.0 and 57.9 ± 5.0 U ml(-1) , respectively. The results indicated that addition of rPON1 during IVM improved embryo development in a dose-dependent manner as D7 embryo development was 22.2%, 29.4%, 32.2% and 37.0% for the treatment groups, respectively (p = 0.02). In conclusion, addition of PON1 enzyme during IVM exerted dose-related positive effects on embryo development rates to blastocysts.

Characterization of the recombinant Brettanomyces anomalus ß-glucosidase and its potential for bioflavouring.

AIM: Plant materials used in the food industry contain up to five times more aromas bound to glucose (glucosides) than free, unbound aromas, making these bound aromas an unused flavouring potential. The aim of this study was to identify and purify a novel ß-glucosidase from Brettanomyces yeasts that are capable of releasing bound aromas present in various food products. METHODS AND RESULTS: We screened 428 different yeast strains for ß-glucosidase activity and are the first to sequence the whole genome of two Brettanomyces yeasts (Brettanomyces anomalus and Brettanomyces bruxellensis) with exceptionally high ß-glucosidase activity. Heterologous expression and purification of the identified B. anomalus ß-glucosidase showed that it has an optimal activity at a higher pH (5·75) and lower temperature (37°C) than commercial ß-glucosidases. Adding this B. anomalus ß-glucosidase to cherry beers and forest fruit milks resulted in increased amounts of benzyl alcohol, eugenol, linalool and methyl salicylate compared to Aspergillus niger and Almond glucosidase. CONCLUSIONS: The newly identified B. anomalus ß-glucosidase offers new possibilities for food bioflavouring. SIGNIFICANCE AND IMPACT OF THE STUDY: This study is the first to sequence the B. anomalus genome and to identify the ß-glucosidase-encoding genes of two Brettanomyces species, and reports a new bioflavouring enzyme.

Development and application of a quantitative method based on LC-QqQ MS/MS for determination of steviol glycosides in Stevia leaves.

Stevia is a currently well-known plant thanks to the presence of steviol glycosides, which are considered as sweeteners obtained from a natural source. In this research, a method based on LC-MS/MS by using a triple quadrupole detector was developed for quantitation of 8 steviol glycosides in extracts from Stevia leaves. The ionization and fragmentation parameters for selected reaction monitoring were optimized. Detection and quantitation limits ranging from 0.1 to 0.5ng/mL and from 0.5 to 1ng/mL, respectively, were achieved: the lowest attained so far. The steviol glycosides were quantified in extracts from leaves of seven varieties of Stevia cultivated in laboratory, greenhouse and field. Plants cultivated in field presented higher concentration of steviol glycosides than those cultivated in greenhouse. Thus, the way of cultivation clearly influences the concentration of these compounds. The inclusion of branches together with leaves as raw material was also evaluated, showing that this inclusion modifies, either positively or negatively, the concentration of steviol glycosides.

Pharmacological targeting of histamine H4 receptor in periodontal disease.

OBJECTIVE: The objective of this study was to investigate whether histamine H4 receptor (H4 R) antagonists could prevent experimental periodontitis (EP)-induced histological, functional and inflammatory alterations in submandibular gland (SMG), periodontal bone and gingiva. METHODS: Bilateral EP was induced for 2 weeks in anaesthetized male rats. The effect of systemic and local administration of H4 R antagonists (JNJ7777120, JNJ10191584) on histopathology and functionality of SMG, bone loss and gingival inflammation was evaluated. RESULTS: The subcutaneous administration of JNJ7777120 prevented periodontitis-induced SMG histological injury, reducing vacuolization and apoptosis and additionally reversed the increased prostaglandin E2 (PGE2) levels in SMG while it partially reversed the methacholine-induced salivation reduction produced by periodontitis. JNJ7777120 attenuated bone loss and the increased PGE2 levels and inflammatory infiltration in gingival tissue of rats with periodontitis. Finally, local administration of JNJ7777120 and JNJ10191584 was also beneficial for improving periodontal parameters. CONCLUSIONS: H4 receptor antagonists are able to ameliorate periodontitis-induced injury on SMG, gingival tissue and bone structure, suggesting that pharmacological targeting of H4 R could be an attractive strategy to improve periodontal health.

The thyroid status reprograms T cell lymphoma growth and modulates immune cell frequencies.

UNLABELLED: In spite of considerable evidence on the regulation of immunity by thyroid hormones, the impact of the thyroid status in tumor immunity is poorly understood. Here, we evaluated the antitumor immune responses evoked in mice with different thyroid status (euthyroid, hyperthyroid, and hypothyroid) that developed solid tumors or metastases after inoculation of syngeneic T lymphoma cells. Hyperthyroid mice showed increased tumor growth along with increased expression of cell cycle regulators compared to hypothyroid and control tumor-bearing mice. However, hypothyroid mice showed a higher frequency of metastases than the other groups. Hyperthyroid mice bearing tumors displayed a lower number of tumor-infiltrating T lymphocytes, lower percentage of functional IFN-γ-producing CD8(+) T cells, and higher percentage of CD19(+) B cells than euthyroid tumor-bearing mice. However, no differences were found in the distribution of lymphocyte subpopulations in tumor-draining lymph nodes (TDLNs) or spleens among different experimental groups. Interestingly, hypothyroid TDLN showed an increased percentage of regulatory T (Treg) cells, while hyperthyroid mice displayed increased number and activity of splenic NK cells, which frequency declined in spleens from hypothyroid mice. Moreover, a decreased number of splenic myeloid-derived suppressor cells (MDSCs) were found in tumor-bearing hyperthyroid mice as compared to hypothyroid or euthyroid mice. Additionally, hyperthyroid mice showed increased cytotoxic activity, which declined in hypothyroid mice. Thus, low levels of intratumoral cytotoxic activity would favor tumor local growth in hyperthyroid mice, while regional and systemic antitumor response may contribute to tumor dissemination in hypothyroid animals. Our results highlight the importance of monitoring the thyroid status in patients with T cell lymphomas. KEY MESSAGES: T cell lymphoma phenotype is paradoxically influenced by thyroid status. Hyperthyroidism favors tumor growth and hypothyroidism rises tumor dissemination. Thyroid status affects the distribution of immune cell types in the tumor milieu. Thyroid status also modifies the nature of local and systemic immune responses.

Muerte de un pasajero en un vuelo comercial. Muerte inesperada por colangitis supurada / Death of a passenger on a commercial flight. Unexpected death from pyogenic cholangitis

Un pasajero de un vuelo comercial desde un país del Magreb a otro del norte de Europa fallece durante el vuelo. El avión aterriza en el aeropuerto más próximo en ese momento, que es el de nuestra ciudad. Comunicado el hecho a la autoridad judicial local, esta ordena la práctica de la autopsia médico-legal. En el presente caso no se pudo conseguir ninguna información de los antecedentes patológicos del fallecido antes de la autopsia. Esta reveló la existencia de una colangitis supurada, debida a litiasis residual del colédoco, como causa de la muerte (AU)

A passenger on a commercial flight from a country in Maghreb to another one in Northern Europe dies during the flight. The plane landed at Malaga Airport (that was the nearest airport at that moment). Statements made to the local judicial authorities ordered the practice of a forensic autopsy. In this case no medical history information from the deceased before the autopsy could be gathered. This revealed the existence of a pyogenic cholangitis due to residual bile duct stones, as the cause of death (AU)